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• #Phy304 vector sequence in snapgene viewer download#

Vector backbone pJet1.2 (Search Vector Database) Backbone manufacturer Thermo scientific Backbone size w/o insert (bp) 2974 Total vector size (bp) 3745 Vector type Golden gate donor vector for gene targeting in Bacillus subtilis Growth in Bacteria Bacterial Resistance (s) Ampicillin Growth Temperature 37☌ Growth Strain (s) DH5alpha Copy number but for longer fragments genes I repeated many times, still not a good result through. For the short fragments insertion, I got a positive result for the first time. Anyone familiar with cloning with pJET 1.2?.‪Vector Addition‬ - PhET Interactive Simulations ‪Vector Addition‬ - PhET Interactive Simulations.Brand: Thermo Scientific™ Price Range: $153 - $289 Offer Count: 2 As a result, only bacterial cells with recombinant plasmids are able to form colonies.

The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. The CloneJET PCR Cloning Kit contains a novel, ready-to-use positive selection cloning vector pJET1.2/blunt. Experiment with vector equations and compare vector sums and differences. Specify vectors in Cartesian or polar coordinates, and see the magnitude, angle, and components of each vector.

#Phy304 vector sequence in snapgene viewer download#

PJET1.2 vector Sequence Copy Sequence Download GeneBank File (.gb) LOCUS Exported 2974 bp ds-DNA circular SYN 03-11-2013 DEFINITION Positive selection cloning vector with a lethal insert that allows for efficient recovery of blunt-ended PCR products. Map and Features of pJET1.2/blunt Cloning Vector Available with the CloneJET™ PCR Cloning Kit (#K1231, #K1232) Enzymes produced by Fermentas are shown in orange. Map and Features of pJET1.2/blunt Cloning Vector.Sequence Author: Thermo Fisher (Fermentas) Positive selection cloning vector with a lethal insert that allows for efficient recovery of blunt-ended PCR products.